Customization: | Available |
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CAS No.: | 77-86-1 |
Formula: | C4h11no3 |
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Product Name: | Tris(hydroxymethyl)aminomethane |
CAS: | 77-86-1 |
MF: | C4H11NO3 |
MW: | 121.14 |
EINECS: | 201-064-4 |
Melting point | 167-172 °C(lit.) |
Boiling point | 219-220 °C10 mm Hg(lit.) |
density | 1,353 g/cm3 |
vapor pressure | 0.0267 hPa (20 °C) |
refractive index | 1.4170 (estimate) |
Fp | 219-220°C/10mm |
storage temp. | 20-25°C |
solubility | H2O: 4 M at 20 °C, clear, colorless |
form | crystalline |
color | white |
PH | 10.5-12.0(4 m in water, 25 °C) |
PH Range | 7 - 9 |
pka | 8.1(at 25ºC) |
Water Solubility | 550 g/L (25 ºC) |
λmax | λ: 260 nm Amax: 0.10 λ: 280 nm Amax: 0.08 |
Sensitive | Hygroscopic |
Merck | 14,9772 |
BRN | 741883 |
Stability: | Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture. |
Application:Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
Tris(hydroxymethyl)aminomethane is used as a buffer in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis. It acts as a precursor for the preparation of polymers, oxazolones and oxazolidines.