Customization: | Available |
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CAS No.: | 593-84-0 |
Formula: | CH5n3.Chns |
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Product Name: | Guanidine thiocyanate |
CAS: | 50-01-1 |
MF: | CH5N3.ClH |
MW: | 95.53 |
EINECS: | 200-002-3 |
CAS: | 593-84-0 |
MF: | CH5N3.CHNS |
MW: | 118.16 |
EINECS: | 209-812-1 |
Melting point | 120-122 °C(lit.) |
density | 1.103 g/mL at 20 °C |
vapor pressure | 0Pa at 20ºC |
refractive index | n20/D 1.482 |
storage temp. | 20-25°C |
solubility | H2O: 6 M at 20 °C, clear, colorless |
form | Crystals or Crystalline Powder |
pka | 12.5[at 20 ºC] |
color | White or colorless |
Specific Gravity | 1.152 |
Odor | Odorless |
PH | 4.8-6.0 (1420g/l, H2O, 20ºC)(saturated solution) |
PH Range | 4.8 - 6.0 |
Water Solubility | Soluble in water and ethanol |
Sensitive | Light Sensitive |
Stability: | Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents. |
Application:Guanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published.
In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost.